Optical Properties of Glycated and Non-Glycated Hemoglobin – Raman/Fluorescence Spectroscopy and Refractometry

In this study, the optical properties of glycated (HbA1c) and non-glycated (Hb) hemoglobin are compared using Surface-enhanced Raman spectroscopy (SERS), spectrofluorimetry, refractometry. Analysis spectral shift SERS spectra showed good discrimination between two hemoglobins indicating differences in their molecular structure. The fluorescence measured at excitation wavelengths 260, 270, 280 nm also indicate structure these hemoglobins. For first time refractive index temperature increments were for HbA1c a wide wavelength range visible NIR as –(1.35 ± 0.11) x 10–4 °C–1 with normal (dn/dT = –(1.02 0.12) °C–1). comparison RI obtained from whole blood healthy volunteers diabetic patients is done. data can serve basis further study other proteins.